Translational_Unit

Part:BBa_K112120:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


rbs.BamHI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

PCR mv031/mv006 on pBca9145-I716210    (692bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112120
-----------------------------------------------
mv031	Forward Biobricking of rbs.r.BamH1!
cgataGAATTCatgAGATCTtaagtgggggcaaattagaatggaagtagaaaaagagtttattactgatgaagc
mv006	Reverse Biobricking of r.BamH1!
cgttaGGATCCctatttgttttcaactttatctttcc

References